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Image Search Results
Journal: Frontiers in medicine
Article Title: Multimodal measurement of glycocalyx degradation during coronary artery bypass grafting.
doi: 10.3389/fmed.2022.1045728
Figure Lengend Snippet: FIGURE 2 A schematic overview of the glycocalyx under physiological (left) and diseased (right) conditions covering the endothelial cells in the lumen of a blood vessel. The perfused boundary region (PBR) is defined as the distance between the median red blood cell (RBC) column width and the limit where erythrocytes can be found. During physiological conditions, the glycocalyx is composed of syndecan-1 and glycosaminoglycans including heparan sulfate (HS) and hyaluronan (HA). Receptors such as thrombomodulin (TM), Tie-2, and E-selectin are present on the endothelial membrane. During disease, the glycocalyx is damaged and components are shed to the plasma, which causes an increase in the PBR as erythrocytes are able to penetrate deeper in the glycocalyx layer. Activation of endothelial cells causes both activation and release of surface receptors TM, Tie-2, and E-selectin. E-selectin activation increases leukocyte rolling, increased Ang-2 levels binding to Tie-2 increases vessel permeability. Figure created with www.biorender.com.
Article Snippet: To determine glycocalyx shedding products, commercial enzymelinked immunosorbent assays (ELISAs) were used according to the manufacturer’s instructions for Syndecan-1 (DuoSet ELISA, R&D systems, Bio-Techne, Minneapolis, USA),
Techniques: Membrane, Clinical Proteomics, Activation Assay, Binding Assay, Permeability
Journal: Frontiers in medicine
Article Title: Multimodal measurement of glycocalyx degradation during coronary artery bypass grafting.
doi: 10.3389/fmed.2022.1045728
Figure Lengend Snippet: FIGURE 3 Boxplot of the perfused boundary region [PBR; (A)], Syndecan-1 (B), heparan sulfate (C), hyaluronan (D), Angiopoietin-2 [Ang-2, (E)], Tie-2 (F), E-selectin (G), and thrombomodulin (H) for all time points. N = 23 during surgery; N = 19 at ICU. ∗p < 0.05.
Article Snippet: To determine glycocalyx shedding products, commercial enzymelinked immunosorbent assays (ELISAs) were used according to the manufacturer’s instructions for Syndecan-1 (DuoSet ELISA, R&D systems, Bio-Techne, Minneapolis, USA),
Techniques: